人IL-17 ELISA试剂盒(Interleukin-17) (ab100556)

概述

  • 产品名称人IL-17 ELISA试剂盒(Interleukin-17)
    参阅全部 IL17 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    15.63 pg/ml - 1000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 93.65 84% - 103%
    Serum 94.15 82% - 103%
    Plasma 92.24 82% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-17 (Interleukin-17) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • 组织特异性Restricted to activated memory T-cells.
  • 序列相似性Belongs to the IL-17 family.
  • 翻译后修饰Found both in glycosylated and nonglycosylated forms.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-17 ELISA Kit (Interleukin-17) 图像

  • Representative standard curve using ab100556

  • Representative standard curve using ab100556

实验方案

Human IL-17 ELISA Kit (Interleukin-17) (ab100556)参考文献

ab100556 has not yet been referenced specifically in any publications.

Product Wall

This kit recognizes IL-17A and does not cross react with IL-17B, C, D, E, or F. Whether the kit will also recognize the IL-17A/F heterodimer has not been tested but some degree of cross reactivity is possible based on sequence similarity.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"