人IL-13 Receptor alpha ELISA试剂盒(ab46066)

概述

  • 产品名称人IL-13 Receptor alpha ELISA试剂盒
    参阅全部 IL13R alpha2 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 14 3024pg/ml 93.67 3.1%
    B 14 1054pg/ml 29.69 2.82%
    批次间
    样品 n Mean SD CV%
    A 8 2744pg/ml 90.1 3.28%
    B 8 725pg/ml 43.9 6.06%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 150 pg/ml
  • 范围
    156 pg/ml - 5000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 90 156pg/ml - 5000pg/ml

  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性The Interleukin-13 receptor alpha-2 chain (IL3Ralpha2) belongs to the type I cytokine receptor family. It binds as a monomer with high affinity to interleukin 13, but lacks a cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.
  • 细胞定位Single-pass type I membrane protein
  • 别名
    • CD213A2
    • IL 13R
    • IL13BP
    • IL13R
    • Interleukin 13 binding protein
    • interleukin 13 receptor, alpha 2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-13 Receptor alpha ELISA Kit 图像

  • Representative Standard Curve using ab46066

实验方案

Human IL-13 Receptor alpha ELISA Kit (ab46066)参考文献

ab46066 has not yet been referenced specifically in any publications.

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