人IL-13 Receptor alpha ELISA试剂盒(ab46066)

概述

  • 产品名称
    人IL-13 Receptor alpha ELISA试剂盒
    参阅全部 IL13 receptor alpha 2 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 14 3024pg/ml 93.67 3.1%
    B 14 1054pg/ml 29.69 2.82%
    批次间
    样品 n Mean SD CV%
    A 8 2744pg/ml 90.1 3.28%
    B 8 725pg/ml 43.9 6.06%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 150 pg/ml
  • 范围
    156 pg/ml - 5000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 90 156pg/ml - 5000pg/ml

  • 检测时间
    3h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-13 Receptor alpha Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-13 Receptor alpha Microplate (12 x 8 well strips) 1 unit 2 units
    IL-13 Receptor alpha Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 功能
    Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
  • 序列相似性
    Belongs to the type I cytokine receptor family. Type 5 subfamily.
    Contains 1 fibronectin type-III domain.
  • 结构域
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • Cancer/testis antigen 19
    • CD213a2
    • CT19
    • I13R2_HUMAN
    • IL-13 receptor subunit alpha-2
    • IL-13R subunit alpha-2
    • IL-13R-alpha-2
    • IL-13RA2
    • IL13 receptor
    • IL13BP
    • IL13R
    • Il13ra2
    • Interleukin 13 binding protein
    • Interleukin 13 receptor alpha 2 chain
    • Interleukin-13 receptor subunit alpha-2
    • Interleukin-13-binding protein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46066

实验方案

文献

ab46066 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab46066.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册