人IL-12 p70 ELISA试剂盒(Interleukin-12 p70) (ab46143)

概述

  • 产品名称
    人IL-12 p70 ELISA试剂盒(Interleukin-12 p70)
    参阅全部 IL12 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.3%
    批次间
    样品 n Mean SD CV%
    Overall 6 8.9%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 2.2 pg/ml
  • 范围
    6.25 pg/ml - 200 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 91 86% - 94%

  • 检测时间
    3h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-12 p70 (Interleukin- 12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 in supernatants, buffered solutions or serum and plasma samples.

    A monoclonal antibody specific IL-12 p70 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 p70 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 p70 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 p70 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-12 p70.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab46143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

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    Representative Standard Curve using ab46143

实验方案

文献

ab46143 has not yet been referenced specifically in any publications.

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