人IL-12 p70 ELISA试剂盒(Interleukin-12 p70) (ab119532)

概述

  • 产品名称
    人IL-12 p70 ELISA试剂盒(Interleukin-12 p70)
    参阅全部 IL12 p70 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 3%
    批次间
    样品 n Mean SD CV%
    Overall 8 4.8%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    2.1 pg/ml
  • 范围
    3.1 pg/ml - 200 pg/ml
  • 复检

    103 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 103 79% - 118%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-12 p70 (Interleukin-12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-12 p70 concentrations in Cell culture supernatant serum, and plasma (EDTA, citrate, heparin).


    Human IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-12 p70 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-12 p70 captured on the plate.


    Get results in 90 minutes with Human IL-12p40/p70 ELISA Kit (ab178009) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human IL12 p70 monoclonal antibody 1 x 100µl
    Control high 1 vial
    Control low 1 vial
    IL-12 p70 Standard, lyophilized (400 pg/mL on reconstitution) 2 vials
    Microplate coated with antibody to Human IL-12 p70 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 序列相似性
    Belongs to the IL-6 superfamily.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119532.

实验方案

文献

ab119532 has not yet been referenced specifically in any publications.

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