人IL-12 p70 ELISA试剂盒(Interleukin-12 p70) (ab100552)

概述

  • 产品名称人IL-12 p70 ELISA试剂盒(Interleukin-12 p70)
    参阅全部 IL12 p70 试剂盒
  • 检测方法Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    0.8 pg/ml - 600 pg/ml
  • 复检

    88 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.52 88% - 109%
    Plasma 85.63 79% - 101%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-12 p70 (Interleukin-12 p70) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in plasma and cell culture supernatants.


     This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-12 p70 in normal serum with ab100552, only in serum spiked with Human IL-12 p70.


    Get results in 90 minutes with Human IL-12p40/p70 ELISA Kit (ab178009) from our SimpleStep ELISA® range.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-12 p70 ELISA Kit (Interleukin-12 p70) 图像

  • Representative standard curve using ab100552

  • Representative standard curve using ab100552

实验方案

Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab100552)参考文献

ab100552 has not yet been referenced specifically in any publications.

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Thank you for contacting us. Low signal in this case may be the result of incubation time. I would recommend that you increase the incubation period of assay step #2 to overnight. I also recommend that you prepare all reagents as you use them, especial...

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