人IL-12 p70 ELISA试剂盒(Interleukin-12 p70) (ab100552)

概述

  • 产品名称人IL-12 p70 ELISA试剂盒(Interleukin-12 p70)
    参阅全部 IL12 p70 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    0.8 pg/ml - 600 pg/ml
  • 复检

    88 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.52 88% - 109%
    Serum 83.34 78% - 102%
    Plasma 85.63 79% - 101%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-12 p70 (Interleukin-12 p70) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in serum, plasma and cell culture supernatants.

     This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-12 p70 ELISA Kit (Interleukin-12 p70) 图像

  • Representative standard curve using ab100552

  • Representative standard curve using ab100552

实验方案

Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab100552)参考文献

ab100552 has not yet been referenced specifically in any publications.

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Thank you for contacting us. Low signal in this case may be the result of incubation time. I would recommend that you increase the incubation period of assay step #2 to overnight. I also recommend that you prepare all reagents as you use them, especial...

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