人IL-11 ELISA试剂盒(Interleukin-11) (ab100551)

概述

  • 产品名称人IL-11 ELISA试剂盒(Interleukin-11)
    参阅全部 IL11 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    3.29 pg/ml - 800 pg/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 101 89% - 108%
    Serum 97.89 85% - 104%
    Plasma 95.5 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-11 (Interleukin-11) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-11 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Adipogenesis inhibitory factor
    • AGIF
    • IL 11
    • IL-11
    • Il11
    • IL11_HUMAN
    • Interleukin 11
    • Interleukin-11
    • Interleukin11
    • Oprelvekin
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-11 ELISA Kit (Interleukin-11) 图像

  • Representative standard curve using ab100551

  • Representative standard curve using ab100551

实验方案

Human IL-11 ELISA Kit (Interleukin-11) (ab100551)参考文献

ab100551 has not yet been referenced specifically in any publications.

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