人IL-10 RB ELISA试剂盒(Interleukin-10 Receptor beta) (ab100550)

概述

  • 产品名称人IL-10 RB ELISA试剂盒(Interleukin-10 Receptor beta)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    4.12 pg/ml - 3000 pg/ml
  • 复检

    > 100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 129.1 120% - 140%
    Serum 121.9 110% - 129%
    Plasma 104.5 92% - 112%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-10 RB (Interleukin 10 Receptor beta) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10 RB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-10 RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29.
  • 疾病相关Defects in IL10RB are the cause of inflammatory bowel disease type 25 (IBD25) [MIM:612567]. It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • 序列相似性Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CDw210b
    • CRF2 4
    • CRF2-4
    • CRFB4
    • Cytokine receptor class II member 4
    • Cytokine receptor class-II member 4
    • Cytokine receptor family 2 member 4
    • D21S58
    • D21S66
    • I10R2_HUMAN
    • IL-10 receptor subunit beta
    • IL-10R subunit 2
    • IL-10R subunit beta
    • IL-10R2
    • IL-10RB
    • IL10 receptor subunit beta
    • IL10R subunit 2
    • IL10R subunit beta
    • IL10R2
    • IL10RB
    • Interleukin 10 receptor subunit 2
    • Interleukin 10 receptor subunit beta
    • Interleukin-10 receptor subunit 2
    • Interleukin-10 receptor subunit beta
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-10 RB ELISA Kit (Interleukin-10 Receptor beta) 图像

  • Representative standard curve using ab100550

  • Representative standard curve using ab100550

实验方案

Human IL-10 RB ELISA Kit (Interleukin-10 Receptor beta) (ab100550)参考文献

ab100550 has not yet been referenced specifically in any publications.

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