人IL-10 ELISA试剂盒(ab100549)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 2.34 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-10 ELISA试剂盒
参阅全部 IL-10 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 1 pg/ml -
范围
2.34 pg/ml - 150 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 98.37 87% - 110% Serum 101.94 87% - 108% Plasma 102.39 93% - 109% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range.
Produced using a non-baculovirus expression system.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-10 2 vials IL-10 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
组织特异性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
序列相似性
Belongs to the IL-10 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
数据库链接
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
相关产品
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SimpleStep ELISA kits
图片
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IL-10 measured in culture supernatants from control (-) or activated cell lines (dilution range 1/1-1/00), stimulated for 24 hours with 50 ng x mL-1 of PMA (P)(ab120297) and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants (dilution range 1/10-1/1000), from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).
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Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).
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Standard curve (assay diluent A) with background signal subtracted (duplicates; +/- SD).
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Dilution curves (in assay diluent B) of human IL-10 (ab100549) and NIBSC standard (93/722). One ng of standard IL-10 corresponds to 0.94 (+/- 0.08) U NIBSC standard. Background signal subtracted (duplicates; +/- SD).
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Representative standard curve using ab100549
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Representative standard curve using ab100549
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab100549 被引用在 18 文献中.
- Xu W et al. Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection. Front Med (Lausanne) 9:896409 (2022). PubMed: 36160124
- Zhou T et al. microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis. Aging (Albany NY) 13:11061-11082 (2021). PubMed: 33744854
- Flaherty RA et al. Distinct Group B Streptococcus Sequence and Capsule Types Differentially Impact Macrophage Stress and Inflammatory Signaling Responses. Infect Immun 89:N/A (2021). PubMed: 33558317
- Sun W et al. The Prognostic Determinant of Interleukin-10 in Patients with Acute Ischemic Stroke: An Analysis from the Perspective of Disease Management. Dis Markers 2021:6423244 (2021). PubMed: 34336007
- El-Ali Z et al. Evaluation of circulating levels of Interleukin-10 and Interleukin-16 and dietary inflammatory index in Lebanese knee osteoarthritis patients. Heliyon 7:e07551 (2021). PubMed: 34337179