人IL-1 beta ELISA试剂盒(Interleukin-1 beta) (ab46052)

概述

  • 产品名称
    人IL-1 beta ELISA试剂盒(Interleukin-1 beta)
    参阅全部 IL1 beta 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    6 4.5%
    批次间
    样品 n Mean SD CV%
    6 8.7%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    6.5 pg/ml
  • 范围
    15.6 pg/ml - 500 pg/ml
  • 复检

    102.2 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 102.2 15.6pg/ml - 500pg/ml

  • 检测时间
    3h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-1 beta.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 2 x 96 tests 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 2 x 10ml 1 x 10ml
    Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml
    Biotinylated anti-IL-1 beta Red 2 x 400µl 1 x 400µl
    Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml
    Control Silver 4 vials 2 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-1 beta Microplate (12 x 8 well strips) 2 units 1 unit
    IL1 beta standard Yellow 4 vials 2 vials
    Standard Diluent (Serum) 2 x 7ml 1 x 7ml
    Stop Reagent Black 2 x 11ml 1 x 11ml
    Streptavidin-HRP 4 x 5µl 2 x 5µl
  • 研究领域
  • 功能
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性
    Belongs to the IL-1 family.
  • 结构域
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 别名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).

  • IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and 1 ug x mL-1 LPS (Sigma) for the last 6 hours (P+L).

  • Representative Standard Curve using ab46052

实验方案

文献

This product has been referenced in:
  • Sajid F & Bano S Pro inflammatory interleukins and thyroid function in Naswar (dipping tobacco) users: a case control study. BMC Endocr Disord 16:47 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27515932) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your reply.

If you diluted the controls in the recommended volume of diluent, then you should have ended up with the concentration that is indicated on the control vials. Was there no concentration listed on the vials? If there ...

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Thank you for contacting Abcam.

For all three of those ELISA kits, there are standards and controls (4 vials of control per kit)provided and you should reconstitute those samples in the correct diluent depending on your sample types.
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Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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