人IL-1 beta ELISA试剂盒(Interleukin-1 beta) (ab108865)
概述
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产品名称人IL-1 beta ELISA试剂盒(Interleukin-1 beta)
参阅全部 IL1 beta 试剂盒 -
检测方法Colorimetric
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精确度
批次内 样品 n Mean SD CV% Overall 4.9% 批次间 样品 n Mean SD CV% Overall 7.2% -
样品类型Cell culture supernatant, Serum, Plasma
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检测类型Sandwich (quantitative)
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灵敏度> 3.9 pg/ml
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范围3.91 pg/ml - 250 pg/ml
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检测时间5h 00m
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实验步骤Multiple steps standard assay
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种属反应性与反应: Human
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产品概述
Abcam’s IL-1 beta Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta concentrations in cell culture supernatants, serum and plasma.
An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.
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经测试应用适用于: Sandwich ELISAmore details
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平台Microplate
性能
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存放说明Store at -20°C. Please refer to protocols.
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组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1x Standard diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human IL-1 beta Antibody 1 x 120µl Chromogen Substrate 1 x 8ml IL-1 beta Microplate (12 x 8 well strips) 1 unit IL-1 beta Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究领域
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功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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序列相似性Belongs to the IL-1 family.
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结构域The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
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细胞定位Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
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别名
- Catabolin
- H1
- Hematopoietin 1
see all -
数据库链接
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
应用
Our Abpromise guarantee covers the use of ab108865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent dilution. |
Human IL-1 beta ELISA Kit (Interleukin-1 beta) 图像
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Typical Standard CurveRepresentative Standard Curve using ab108865.
实验方案
Human IL-1 beta ELISA Kit (Interleukin-1 beta) (ab108865)参考文献
ab108865 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"