人IL-1 beta ELISA试剂盒(Interleukin-1 beta) (ab108865)

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概述

  • 产品名称
    人IL-1 beta ELISA试剂盒(Interleukin-1 beta)
    参阅全部 IL1 beta 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    > 3.9 pg/ml
  • 范围
    3.91 pg/ml - 250 pg/ml
  • 检测时间
    5h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Human IL-1 beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta concentrations in cell culture supernatants, serum and plasma.


    An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human IL-1 beta Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    IL-1 beta Microplate (12 x 8 well strips) 1 unit
    IL-1 beta Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性
    Belongs to the IL-1 family.
  • 结构域
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 别名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab108865 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent dilution.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108865.

    实验方案

    文献

    ab108865 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab108865？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    2 reviews or Q&A

    10
    Votes: Highest First

    Hola,
    Estoy buscando kits de ELISA para detección de citocinas (en particular, IL-1 beta, IL-17 e IFN gamma, pero si van bien, luego podrían ser más), pero necesitaría que detectaran citocinas tanto humanas como de rat&...

    Read More

    Gracias por contactarnos.


    He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

    Tenemos varios kits contra dichas citoquinas espec&iacu...

    Read More

    I have a few questions regarding the following kits:
    Ab48478 - IL6 Human Elipair Kit (Reagents for 5 x 96 Well Plates), 5x 96 testsAb47351 - Human IL1 beta Elipair Kit (Reagents for 5 x 96 Well Plates), 1kit

    1. Are the ELISA plates supp...

    Read More

    Thank you for contacting us.

    1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) an...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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