样品 | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 4.9% |
样品 | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 7.2% |
Human IL-1 beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta concentrations in cell culture supernatants, serum and plasma.
An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.
组件 | 1 x 96 tests |
---|---|
100X Streptavidin-Peroxidase Conjugate | 1 x 80µl |
10X Diluent N Concentrate | 1 x 30ml |
1X Standard Diluent | 1 x 2ml |
20X Wash Buffer Concentrate | 2 x 30ml |
50X Biotinylated Human IL-1 beta Antibody | 1 x 120µl |
Chromogen Substrate | 1 x 8ml |
IL-1 beta Microplate (12 x 8 well strips) | 1 unit |
IL-1 beta Standard | 1 vial |
Sealing Tapes | 3 units |
Stop Solution | 1 x 12ml |
Our Abpromise guarantee covers the use of ab108865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use at an assay dependent dilution. |
ab108865 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"