Anti-Human IgM Fc fragment抗体[CH2] (ab772)

概述

  • 产品名称Anti-Human IgM Fc fragment抗体[CH2]
    参阅全部 Human IgM Fc fragment 一抗
  • 描述
    小鼠单克隆抗体[CH2] to人IgM Fc fragment
  • 特异性This antibody reacts with Fc fragment of human IgM. Specificity was confirmed by Western blotting analysis of purified human IgM, under reducing conditions, and also by immunofluorescence staining of Daudi cells
  • 经测试应用适用于: ICC/IF, Flow Cyt, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by DEAE chromatography and precipitation methods.
  • 克隆单克隆
  • 克隆编号CH2
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Flow Cyt
WB
ELISA
  • 应用说明ELISA: Use at an assay dependant concentration.
    Flow Cyt: Use at an assay dependant concentration.
    IF: Use at an assay dependant concentration.
    WB: Use at an assay dependant concentration.
    This antibody blocks binding of standard antibody to Fc fragment of human IgM and does not block binding of standard antibody to Fab fragment of human IgM.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
    • 细胞定位Cell Membrane and Secreted
    • 数据库链接
    • 别名
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    Anti-Human IgM Fc fragment antibody [CH2] (ab772)参考文献

    ab772 has not yet been referenced specifically in any publications.

    Product Wall

    The originator of these two antibodies has informed me that the anti Fab fragment antibody is better (IgG receptors bind IgG via Fc so it may be occupied). The Fc fragment is not good for IgM bound to the B-cell surface, but is great for soluble IgM.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"