The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/5 - 1/10.
IHC-Fr: 1/5 - 1/10.
IHC-P: 1/5 - 1/10 (based on the Indirect Method). With a normal ABC Method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution. Perform protease digestion trypsin pretreatment. Dilute immediately before use with PBS or TRIS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells.
Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.
IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.