人IgG ELISA试剂盒(ab100547)

概述

  • 产品名称人IgG ELISA试剂盒
    参阅全部 IgG 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 150 pg/ml
  • 范围
    0.021 ng/ml - 15 ng/ml
  • 复检

    117 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 116.1 105% - 123%
    Plasma 118.1 99% - 126%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IgG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG in serum and plasma.

     

    For a more sensitive analysis of human IgG, try our Human SimpleStep ELISA Kit: Quantify as little as 20 pg/mL of human IgG in plasma, serum and other sample types.

     

  • 说明

    The Human immune system consists of two functional components classified as the innate system (the physical, biochemical and cellular barriers), and the adaptive immune system (including lymphocytes and immunoglobulins). Immunoglobulins are the key elements of the humoral immune response in vertebrate against parasitic invasion.

    The polypeptide chains of immunoglobulins composed of two identical heavy (H) chains and two identical light (L) chains linked together by inter-chain disulfide bonds. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membranes. Based upon the variation of the constant region of the heavy chain, nine immunoglobulin heavy chain isotypes are found in humans: IgA (with subclasses IgA1 and IgA2), IgD, IgE, IgM, and IgG (with subclasses IgG1, IgG2, IgG3, and IgG4).

    IgG is the predominant immunoglobulin in the serum, which accounts for 75% of the total serum antibody of healthy individuals. IgG has a molecular weight of about 150 kDa. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960’s by using polyclonal antisera prepared in animals immunized with human myeloma proteins.

    The capture antibody of the kit ab100547 detects the Fc portion of the heavy chain of all subclasses of human IgG and the detection antibody detects the F(ab’)2 fragment of IgG.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
  • 细胞定位Cell Membrane and Secreted
  • 别名
    • Ig gamma
    • Ig gamma-1 chain C region
    • IGHG1
    • Immunoglobulin G
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IgG ELISA Kit 图像

  • Representative standard curve using ab100547

实验方案

Human IgG ELISA Kit (ab100547)参考文献

ab100547 has not yet been referenced specifically in any publications.

Product Wall

IgG levels in human milk smaples by ELISA

Average Average 3/5 (Ease of Use)
Abreviews
Abcam´s IgG human ELISA kit was performed according to manufacturer protocol except to sample preparation, because this protocol was not previously validated for human milk samples. Therefore, we had to dilute for achieve the expected concentrations in samples. For example, we did a dilution of 1: 1,000,000 to get 1-3 mg/mL concentrations (data from scientific studies) at follows:
Add 1 μL of serum human milk into a tube with 99 μL 1X Assay Diluent B to prepare a 100-fold diluted sample. Mix through and then pipette 1 μL of prepared 100-fold diluted sample into a tube with 99 μL 1X Assay Diluent to prepare a 10,000 fold diluted sample. Mix through and then pipette 1 μL of prepared 10,000-fold diluted sample into a tube with 99 μL 1X Assay
More optimized dilutions are required to improve the sensibility of the low concentrations. Moreover, it’s important to point out that these immunoglobulin levels are low in milk samples, unlike serum from blood. It would be advisable optimize the type of diluent and standards to this type of sample. Despite of these reasons, IgG was detected in all samples, showing levels between 0,200 and 500 mg/mL.
In our opinion, it would be necessary positive controls to perform the analysis.
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Abcam user community

Verified customer

提交于 Aug 20 2014

1. I can confirm that the standard included with this kit is total human IgG purified from plasma

2. This kit can detect all IgG subclasses, however we have not determined the relative sensitivity to each.

Epitope mapping has not been performed. However, the capture antibody is a mouse monoclonal and recognizes the Fc portion and the detection antibody is polyclonal and recognizes the Fab portion.

Sample binds to an antibody specific for Human IgG coated on the 96-well plate.

The standard of the kit is total human IgG purified from plasma. We do not sell kit components separately, however the equivalent in our catalogue would be ab98981, www.abcam.com/ab98981.

For the assay diluent I would recommend PBS buffer.

You are right with your calculations where at the end of the Item F preparation, you will have a 8,000uL volume. Since100uL is used for each well, you will have enough for rougly 80 wells. However, there will be some volume lost during pipetting,transf...

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For this kit, there is no need to use positive control. The kit has an anti-Human IgG antibody coated on the well, and you simply need to add your sample (unknown protein amount) together with a fixed concentration of standard protein (Item C).You can ...

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We unfortunately do not have bigger sizes for this kit. You can however order 2 kits at a time, if you are interested in more of these.

The capture antibody of the kit ab100547 detects the Fc portion of the heavy chain of all subclasses of human IgG and the detection antibody detects the F(ab’)2 fragment of IgG.

Indeed, the Human IgG ELISA Kit ab100547 will recognize and detect all IgG subclasses. Based on our QC tests of normal human serum, the concentration level measured with this kit is approximately 12mg/ml (i.e. very high compared to other serum proteins...

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