概述

  • 产品名称Anti-Human IgG抗体(FITC)
    参阅全部 Human IgG 一抗
  • 描述
    山羊多克隆抗体to人IgG (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性Low background fluorescence of normal tissue in contrast to cases of autoimmune disease, deposited immunoglobulin IgG. No cross reactivity with albumin fractions of human serum. No cross reactivity with albumin fractions of animal species.
  • 经测试应用适用于: IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human IgG.

  • 常规说明

    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel filtation and ion exchange chromatography to clear unbound conjugate. F/P ratio: 4-6 mol Fluorescein per 1 mol of goat IgG.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorbed by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/40.
Flow Cyt Use at an assay dependent concentration. ab37394-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Anti-Human IgG antibody (FITC) (ab81051)参考文献

This product has been referenced in:
  • Gramlich OW  et al. Enhanced insight into the autoimmune component of glaucoma: IgG autoantibody accumulation and pro-inflammatory conditions in human glaucomatous retina. PLoS One 8:e57557 (2013). ICC/IF ; Human . Read more (PubMed: 23451242) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab81051.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"