人IGFBP6 ELISA试剂盒(ab100544)

概述

  • 产品名称
    人IGFBP6 ELISA试剂盒
    参阅全部 IGFBP6 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 150 pg/ml
  • 范围
    82.3 pg/ml - 60000 pg/ml
  • 复检

    > 80 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 80.6 70% - 91%
    Serum 81.3 71% - 90%
    Plasma 83.5 72% - 92%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IGFBP6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP6 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IGFBP6 (lyophilized) 2 vials
    IGFBP6 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IGFBP6 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 序列相似性
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 翻译后修饰
    O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • IBP 6
    • IBP-6
    • IBP6
    • IBP6_HUMAN
    • IGF binding protein 6
    • IGF-binding protein 6
    • IGFBP 6
    • IGFBP-6
    • IGFBP6
    • Insulin like growth factor binding protein 6
    • Insulin-like growth factor-binding protein 6
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100544

  • Representative standard curve using ab100544

实验方案

文献

ab100544 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you and your customer for your inquiry.

Although we have not tested the Human ab100540 and ab100544ELISA kits with ascite fluid,these kits are very accommodating to many different samples types so the ascites will most likely work.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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