人IGFBP5 ELISA试剂盒(ab100543)

概述

  • 产品名称人IGFBP5 ELISA试剂盒
    参阅全部 IGFBP5 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 ng/ml
  • 范围
    1.1 ng/ml - 800 ng/ml
  • 复检

    89 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 71.5 65% - 92%
    Serum 94.3 81% - 103%
    Plasma 91.6 70% - 100%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IGFBP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 组织特异性Osteosarcoma, and at lower levels in liver, kidney and brain.
  • 序列相似性Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • IBP 5
    • IBP-5
    • IBP5
    • IBP5_HUMAN
    • IGF binding protein 5
    • IGF BP5
    • IGF-binding protein 5
    • IGFBP 5
    • IGFBP-5
    • IGFBP5
    • Insulin like growth factor binding protein 5
    • Insulin-like growth factor-binding protein 5
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP5 ELISA Kit 图像

  • Representative standard curve using ab100543

实验方案

Human IGFBP5 ELISA Kit (ab100543)参考文献

ab100543 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

For both of these ELISA kits we do recommend that you make the wash buffer up fresh before every use and we haven't tested the long term stability of the buffer when diluted to 1X. It should be good if stored o...

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Thank you for your patient. I just had a response from my lab colleagues. They confirmed that all three kits were designed against the mature form of the protein (which is the full amino acid sequence minus the signalling peptide). As the epito...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"