概述

  • 产品名称人IGFBP1 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    > 92 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.64 83% - 102%
    Serum 95.27 84% - 104%
    Plasma 92.47 82% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IGFBP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Insulin like growth factor binding protein 1 (IGFBP1) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The N terminal and C terminal regions are highly homologous among rat, human and bovine sequences. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner.
  • 别名
    • AFBP
    • hIGFBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • Insulin-like growth factor-binding protein 1
    • Placental protein 12
    • PP12
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP1 ELISA Kit 图像

  • IGFBP1 measured in cell lysates showing quantity (pg) per mg of protein

  • IGFBP1 measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100539

  • Representative standard curve using ab100539

实验方案

Human IGFBP1 ELISA Kit (ab100539)参考文献

This product has been referenced in:
  • Davila J  et al. Rac1 Regulates Endometrial Secretory Function to Control Placental Development. PLoS Genet 11:e1005458 (2015). Read more (PubMed: 26305333) »
  • DeCensi A  et al. Differential effects of metformin on breast cancer proliferation according to markers of insulin resistance and tumor subtype in a randomized presurgical trial. Breast Cancer Res Treat 148:81-90 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25253174) »

See all 3 Publications for this product

Product Wall

The immunogen used to raise the antibody pair in IGFBP1 Human ELISA Kit was a recombinant human IGFBP-1, aa 26-259. (This is the full length protein minus the signal peptide).

Unfortunately, epitope mapping has not been performed so it is not ...

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Abreviews
I tried this product on the conditioned media from the decidualized stromal cells. The product met my expectation and the protocol provided was clear and easy to use.
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Verified customer

提交于 Sep 03 2013

Vielen Dank für Ihren Anruf.

Leider ist es so, wie ich befürchtet habe, wir können leider keine Angabe zu diesen Kits mit HepG2 Zellen, bzw mit anderen Leberzelllinien machen. Grundsätzlich spricht aber nichts gegen die Ver...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"