人IGF1 ELISA试剂盒(ab100545)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.1 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IGF1 ELISA试剂盒
参阅全部 IGF1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.2 ng/ml -
范围
0.1 ng/ml - 30 ng/ml -
回收率
97 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102.5 98% - 110% Serum 119.6 105% - 138% Plasma 105.4 81% - 121% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IGF1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IGF1 in serum, plasma, and cell culture supernatants.
This ELISA kit can only detect the free form of human IGF-1. It does not detect the complex or bound forms such as IGFBP-bound IGF1.
This assay employs an antibody specific for Human IGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml Assay Diluent C 2 x 30ml Biotinylated anti-Human IGF-1 2 vials IGF-1 Microplate (12 strips x 8 wells) 1 unit Recombinant Human IGF1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
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别名
- IBP1
- IGF I
- IGF IA
see all -
数据库链接
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P05019 Human
- SwissProt: P01343 Human
- Unigene: 160562 Human
相关产品
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SimpleStep ELISA kits
图片
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab100545 被引用在 23 文献中.
- Zappelli E et al. α-Glyceryl-phosphoryl-ethanolamine protects human hippocampal neurons from ageing-induced cellular alterations. Eur J Neurosci 56:4514-4528 (2022). PubMed: 35902984
- Pourabbas M et al. Strategic Ingestion of High-Protein Dairy Milk during a Resistance Training Program Increases Lean Mass, Strength, and Power in Trained Young Males. Nutrients 13:N/A (2021). PubMed: 33804259
- Dabasinskaite L et al. The Effect of Ozone Treatment on the Physicochemical Properties and Biocompatibility of Electrospun Poly(e)caprolactone Scaffolds. Pharmaceutics 13:N/A (2021). PubMed: 34452249
- Liu HW et al. Effect of Progressive Resistance Training on Circulating Adipogenesis-, Myogenesis-, and Inflammation-Related microRNAs in Healthy Older Adults: An Exploratory Study. Gerontology 66:562-570 (2020). PubMed: 33022678
- Yu AP et al. Obestatin and growth hormone reveal the interaction of central obesity and other cardiometabolic risk factors of metabolic syndrome. Sci Rep 10:5495 (2020). PubMed: 32218464