概述

  • 产品名称人IGF1 ELISA试剂盒
    参阅全部 IGF1 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.2 ng/ml
  • 范围
    0.1 ng/ml - 30 ng/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 102.5 98% - 110%
    Serum 119.6 105% - 138%
    Plasma 105.4 81% - 121%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IGF1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IGF1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • 疾病相关Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • IBP1
    • IGF I
    • IGF IA
    • IGF IB
    • IGF-I
    • Igf1
    • IGF1_HUMAN
    • IGF1A
    • IGFI
    • IGFIA
    • Insulin like growth factor 1
    • Insulin like growth factor 1 (somatomedin C)
    • Insulin like growth factor IA
    • Insulin like growth factor IB
    • Insulin-like growth factor I
    • Mechano growth factor
    • MGF
    • OTTHUMP00000195080
    • OTTHUMP00000195081
    • OTTHUMP00000195082
    • OTTHUMP00000195083
    • OTTHUMP00000195084
    • Somatomedia C
    • Somatomedin C
    • Somatomedin-C
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IGF1 ELISA Kit 图像

  • Representative standard curve using ab100545.

  • IGF1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-64 fold.

  • Human IGF1 standard curve: mean of duplicates (+/- SD)

实验方案

Human IGF1 ELISA Kit (ab100545)参考文献

ab100545 has not yet been referenced specifically in any publications.

Product Wall

This kit is designed to measure free IGF1. IGF-1 is usually bound to IGFBPs in the serum/plasma, so if you would like to measure total IGF1, it is advisable to release the analytes by performing an extraction procedure such as acid-ethanol treatment (P...

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Unfortunately the immunogen sequence for the antibodies used in the kit ab100545 is not available. Nor do we have cross reactivity data for kangaroo species. If it helps, the capture antibody used is monoclonal and the detection antibody is polyclonal....

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L’anticorps de capture de ce kit ne reconnait pas l’IGF-1 du rat. Pour l’anticorps de détection, la réactivé avec la protéine du rat n’a pas été testé donc malheureusement, ce kit...

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The host species for the capture antibody is mouse and the detection antibody is goat.

Vielen Dank für Ihre Antwort und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"