重组抗人IgA1抗体[RM124] (ab193187)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM124] to Human IgA1
- Suitable for: Sandwich ELISA, ELISA
- Reacts with: Human
概述
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产品名称
抗人IgA1抗体[RM124] -
描述
兔单克隆抗体[RM124] to人IgA1 -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISA, ELISAmore details -
种属反应性
与反应: Human -
免疫原
Full length protein corresponding to Human IgA1.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from an animal origin–free culture supernatant. -
克隆
单克隆 -
克隆编号
RM124 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193187于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use a concentration of 0.05 - 0.2 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use a concentration of 0.05 - 0.2 µg/ml. |
ELISA
Use at an assay dependent concentration. |
靶标
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相关性
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90% and IgA2:10%. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined. -
数据库链接
- Entrez Gene: 3493 Human
- Omim: 146900 Human
- SwissProt: P01876 Human
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别名
- FLJ14473 antibody
- FLJ35065 antibody
- FLJ35500 antibody
see all
图片
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Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody
as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. -
ELISA of Human immunoglobulins (the plate was coated with 50 ng/well of different immunoglobulins) using ab193187 at 200 ng/mL, 50 ng/mL, or 10 ng/mL as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody. The graph shows ab193187 only reacted to Human IgA. There is very slightly cross reactivity with IgA2. No cross reactivity occurs with Human IgG, IgM, IgD, or IgE.
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A titer ELISA of Human IgA1 with ab193187 as the primary antibody. The plate was coated with Human IgA1 at different concentrations then a serial dilution of ab193187 was used as the primary antibody . An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab193187 被引用在 2 文献中.
- Aazami H et al. Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients. Allergy Asthma Clin Immunol 16:30 (2020). PubMed: 32351585
- Onodera T et al. Immune-Focusing Properties of Virus-like Particles Improve Protective IgA Responses. J Immunol 203:3282-3292 (2019). PubMed: 31704880