The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.06µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain.
In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species.
Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
Cell Membrane: single-pass type I membrane protein and Secreted
ab111891 at 0.06 µg/10<> cells detecting Human Ig light chain in Lewis Rat spleen cells by Flow Cytometry. 40% more of the cells were stained than in the control (Mouse IgG2a). Strains Wistar, Brown Norway, Buffalo and Fischer 344 were also positive in this assay.