人IFN gamma ELISA试剂盒(ab46025)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 12.5 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IFN gamma ELISA试剂盒
参阅全部 Interferon gamma 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample 30 4.9% 批次间 样品 n Mean SD CV% Sample 30 10% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 5 pg/ml -
范围
12.5 pg/ml - 400 pg/ml -
回收率
107 %
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检测时间
2h 40m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human interferon gamma ELISA kit is designed for the quantitative measurement of IFN gamma in supernatants, buffered solutions or serum and plasma samples and other body fluids.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant Human IFN gamma.
Get higher sensitivity in only 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml 1 x 7.5ml 1 x 13ml Biotinylated anti-IFN gamma Red 1 x 400µl 2 x 400µl 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml 1 x 12ml 1 x 23ml IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units 1 unit 2 units IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials 2 vials 4 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl 2 x 5µl 4 x 5µl -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
相关产品
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Related Products
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SimpleStep ELISA kits
图片
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Representative Standard Curve using ab46025
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab46025 被引用在 15 文献中.
- Ye T et al. LncRNA CCAT1 is overexpressed in tuberculosis patients and predicts their survival. Immun Inflamm Dis 10:218-224 (2022). PubMed: 34847295
- Long C et al. B7-H3 as a Target for CAR-T Cell Therapy in Skull Base Chordoma. Front Oncol 11:659662 (2021). PubMed: 34868903
- Yang L et al. Long non-coding RNA GAS5 protects against Mycoplasma pneumoniae pneumonia by regulating the microRNA-222-3p/TIMP3 axis. Mol Med Rep 23:N/A (2021). PubMed: 33760178
- Ye Y et al. miR-489-3p inhibits TLR4/NF-?B signaling to prevent inflammation in psoriasis. Exp Ther Med 22:744 (2021). PubMed: 34055060
- Zhao J et al. Optimization of GPC3-specific chimeric antigen receptor structure and its effect on killing hepatocellular carcinoma cells. Bioengineered 12:3674-3683 (2021). PubMed: 34261411