人ICAM3 ELISA试剂盒(CD50) (ab46024)

概述

  • 产品名称人ICAM3 ELISA试剂盒(CD50)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 19.75ng/ml 0.172 0.86%
    Sample B 8 2.83ng/ml 0.099 3.49%
    批次间
    样品 n Mean SD CV%
    Sample A 36 19.51ng/ml 0.391 1.99%
    Sample B 36 2.71ng/ml 0.176 6.27%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.3 ng/ml
  • 范围
    0.62 ng/ml - 20 ng/ml
  • 检测时间
    1h 40m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s ICAM3 (CD50) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM3 in serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM3 present in the samples. 

    This kit will recognize both endogenous and recombinant Human ICAM3.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-ICAM-3 (CD50) Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    ICAM3 Control Silver 2 vials 4 vials
    ICAM3 Microplate (12 x 8 well strips) 1 unit 2 units
    ICAM3 Standard Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性ICAM3 is a member of the intercellular adhesion molecule family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule.
  • 细胞定位Cell Membrane; single-pass type I membrane protein
  • 别名
    • CD50
    • CDW50
    • ICAM 3
    • ICAM R
    • ICAMR
    • Intercellular adhesion molecule 3
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human ICAM3 ELISA Kit (CD50) 图像

  • Representative Standard Curve using ab46024

实验方案

Human ICAM3 ELISA Kit (CD50) (ab46024)参考文献

ab46024 has not yet been referenced specifically in any publications.

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The expiration date that we assigned for that lot is October 2013. However, our guarantee is in effect for only 6 months after the purchase of the kit, so I recommend contacting us immediately if you have any iss...

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The lab let me know that it is not necessary to use glass vials. Plastic vials suit perfectly. The crucial point is cleanness.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"