概述

  • 产品名称人ICAM2 ELISA试剂盒(CD102)
    参阅全部 ICAM2 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 23.62U/ml 0.277 1.17%
    B 8 2.96U/ml 0.86 2.9%
    批次间
    样品 n Mean SD CV%
    A 33 23.26U/ml 0.668 2.87%
    B 33 3.12 0.218 6.98%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.2 U/ml
  • 范围
    0.75 U/ml - 24 U/ml
  • 检测时间
    1h 40m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human ICAM2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble Inter Cellular Adhesion Molecule -2 (ICAM2) in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for ICAM2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM2 present in the samples.

    This kit will recognize both endogenous and recombinant Human ICAM2.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • 细胞定位Type 1 membrane protein
  • 别名
    • CD102
    • ICAM 2
    • Intercellular adhesion molecule 2
    • Ly60
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human ICAM2 ELISA Kit (CD102) 图像

  • Representative Standard Curve using ab46080

实验方案

Human ICAM2 ELISA Kit (CD102) (ab46080)参考文献

ab46080 has not yet been referenced specifically in any publications.

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