人I-309 ELISA试剂盒(CCL1) (ab100536)

概述

  • 产品名称人I-309 ELISA试剂盒(CCL1)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    4.12 pg/ml - 3000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 79.9 70% - 103%
    Serum 106.9 97% - 116%
    Plasma 97.65 88% - 110%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in serum, plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and
    I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • BF534335
    • C C motif chemokine 1
    • C-C motif chemokine 1
    • Ccl1
    • CCL1_HUMAN
    • CCR8 ligand
    • Chemokine CC Motif Ligand 1
    • Inflammatory cytokine I-309
    • P500
    • RP23-446K18.2
    • SCYA1
    • SISe
    • Small inducible cytokine A1
    • Small-inducible cytokine A1
    • T lymphocyte secreted protein I-309
    • T lymphocyte-secreted protein I-309
    • TCA3
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human I-309 ELISA Kit (CCL1) 图像

  • Representative standard curve using ab100536

实验方案

Human I-309 ELISA Kit (CCL1) (ab100536)参考文献

ab100536 has not yet been referenced specifically in any publications.

Product Wall

CCL1-I309 human macrophages and DC secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL1-I309 secretion by human macrophages and DC isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr.
good specificity as shown by CCL1 secretion in all macrophages subtypes (M0=MCSF M1=MCSF+IFNg M2a=MCSF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb) and DC (GMCSF+IL4) on day 3 & 7.
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提交于 Apr 09 2014

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I am sorry that this product did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to cont...

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Thank you for forwarding your customer's data. We would like to offer some tips to improve the results seen with the standard curve.For clarity, I have separated the evaluation of the data by the two experiments.


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