人HVEM ELISA试剂盒(TNFRSF14 ) (ab113335)

概述

  • 产品名称
    人HVEM ELISA试剂盒(TNFRSF14 )
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    32.77 pg/ml - 8000 pg/ml
  • 复检

    88 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 85.34 69% - 98%
    Serum 90.17 81% - 97%
    Plasma 90.83 73% - 112%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s HVEM Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HVEM in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human HVEM coated on a 96- well plate. Standards and samples are pipetted into the wells and HVEM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HVEM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HVEM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human HVEM 2 vials
    HVEM Microplate (12 strips x 8 wells) 1 x 96 tests
    Recombinant Human HVEM Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
  • 组织特异性
    Widely expressed, with the highest expression in lung, spleen and thymus.
  • 序列相似性
    Contains 3 TNFR-Cys repeats.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • CD270
    • Herpes virus entry mediator A
    • Herpesvirus entry mediator A
    • HveA
    • TNFRSF14
    • TNR14_HUMAN
    • TR2
    • Tumor necrosis factor receptor superfamily member 14
    • Tumor necrosis factor receptor-like 2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab113335

实验方案

文献

ab113335 has not yet been referenced specifically in any publications.

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