人HSP60 ELISA试剂盒(ab124534)

概述

  • 产品名称人HSP60 ELISA试剂盒
    参阅全部 HSP60 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4 2.1%
    批次间
    样品 n Mean SD CV%
    Overall 3 6.7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 产品概述

    ab124534 HSP60 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of HSP60 protein a 60 kDa protein encoded by the Human HSPD1 gene, in cell and tissue lysates. The assay employs an HSP60 specific antibody coated onto well plate strips.

    Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-HSP60 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.

    Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

     

    Species– Human reactive. Non-reactive with mouse and rat. Others untested.

  • 说明

    Typical working ranges:

    HepG2 cell extract: 0.39 - 200 µg/mL

    Hela cell extract: 3.125 - 200 µg/mL

    Store all components at 4°C. This kit is stable for at least 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab124534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human HSP60 ELISA Kit 图像

  • Example standard curve of serially titrated HepG2 cell lysate in the working range of the assay.
  • Example standard curve of serially titrated Hela cell lysate in the working range of the assay.
  • Detection of endogenous HSP60 levels in different cell lysates using Western blot. HSP60 antibody at 1µg/mL: HepG2 cell lysate (lane 2), HeLa cell lysate (lane 3), and HDFn lysate (lane 4), each were loaded at 15µg/lane.
  • Detection of endogenous HSP60 levels in different cell lysates using HSP60 ELISA kit.
  • Sensitivity of HSP60 ELISA kit is used to demonstrate the quantitative differentiation among different human cell lines.
  • Western blot using the HSP60 detector antibody at 1µg/mL demonstrates assay specificity. HepG2 cell lysate loaded at 15µg/lane.
  • Analysis of HSP60 staining in HDFn cells by ICC: HSP60 primary antibody at 1 µg/mL (green).
  • Analysis of HSP60 staining in Hela cells by Flow: blue: 1µg/mL HSP60 antibody; red: an equal amount of an isotype control antibody.

实验方案

Human HSP60 ELISA Kit (ab124534)参考文献

ab124534 has not yet been referenced specifically in any publications.

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I've confirmed with the lab that the kit has been tested in a spike-recovery assay using 10% goat serum but not with human serum. The average recovery ratewas ˜78%...

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