概述

  • 产品名称人Hemopexin ELISA试剂盒(HPX)
    参阅全部 Hemopexin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.3%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 6 ng/ml
  • 范围
    6.25 ng/ml - 400 ng/ml
  • 复检

    98 %

  • 检测时间
    3h 30m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human cell culture supernatants, milk, saliva and urine.

    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 序列相似性Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • 翻译后修饰N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Beta 1B glycoprotein
    • Beta-1B-glycoprotein
    • FLJ56652
    • HEMO
    • HEMO_HUMAN
    • Hemopexin
    • Hpx
    • HX
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Hemopexin ELISA Kit (HPX) 图像

  • Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.

  • Representative Standard Curve using ab108860.

实验方案

Human Hemopexin ELISA Kit (HPX) (ab108860)参考文献

This product has been referenced in:
  • Zager RA  et al. Renal cortical hemopexin accumulation in response to acute kidney injury. Am J Physiol Renal Physiol 303:F1460-72 (2012). Sandwich ELISA . Read more (PubMed: 22993068) »

See 1 Publication for this product

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