概述

  • 产品名称
    人Hemopexin ELISA试剂盒(HPX)
    参阅全部 Hemopexin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.3%
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 6 ng/ml
  • 范围
    6.25 ng/ml - 400 ng/ml
  • 复检

    98 %

  • 检测时间
    3h 30m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human cell culture supernatants, milk, saliva and urine.


    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.


    Get higher sensitivity in only 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.

  • Representative Standard Curve using ab108860.

实验方案

文献

This product has been referenced in:
  • Zager RA  et al. Renal cortical hemopexin accumulation in response to acute kidney injury. Am J Physiol Renal Physiol 303:F1460-72 (2012). Sandwich ELISA . Read more (PubMed: 22993068) »

See 1 Publication for this product

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