人Hemopexin ELISA试剂盒(HPX) (ab108859)

概述

  • 产品名称人Hemopexin ELISA试剂盒(HPX)
    参阅全部 Hemopexin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.4%
  • 样品类型
    Milk, Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    > 0.7 µg/ml
  • 范围
    0.078 µg/ml - 5 µg/ml
  • 复检

    98 %

  • 检测时间
    2h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.


    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 序列相似性Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • 翻译后修饰N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Beta 1B glycoprotein
    • Beta-1B-glycoprotein
    • FLJ56652
    • HEMO
    • HEMO_HUMAN
    • Hemopexin
    • Hpx
    • HX
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Human Hemopexin ELISA Kit (HPX) 图像

  • Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample

  • Representative Standard Curve using ab108859.

实验方案

Human Hemopexin ELISA Kit (HPX) (ab108859)参考文献

This product has been referenced in:
  • Evans C  et al. Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1. Haematologica N/A:N/A (2015). Read more (PubMed: 26315932) »
  • Insenser M  et al. Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study. PLoS One 9:e100902 (2014). Read more (PubMed: 24979072) »

See all 2 Publications for this product

Product Wall

Hemopexin (HPX) Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: EDTA human plasma stored at -80ºC
Amount of Sample: 3 l
Type of Kit Used: ELISA
Manufacturer instructions were followed.
Additional data
We diluted 3 l of plasma in 1200 l of 1XDiluent N (1:400)
Washings were done on a Microplate Washer machine with 300 l of wash buffer.
The standards data generated an excellent curve.
All samples assayed showed data inside the curve.
Username

Abcam user community

Verified customer

提交于 Apr 08 2015

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

Read More

Thank you for your enquiry.

Sorry for the confusion here. A large excess of Standard is provided in this ELISA kit. To properly dilute the standard, just reconstitute the 3.5ug Standard you received in 0.7ml of Diluent to produce a 5ug/ml Sta...

Read More

Abreview for Hemopexin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum
Amount of Sample: 2 ul µg
Type of Kit Used: ELISA
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: We diluted 2ul of plasma with 800 ul of Diluent (1:400).
We washed the plate 6 times with a machine.
The standards data generated an excelent curve with a fitting error of
3.48e-5.
All samples assayed showed data inside the curve.
Username

Dr. Rafael Montes Nieto

Verified customer

提交于 Mar 26 2012

Thank you for your phone call. Please find testing data for ab108859 attached. I hope this helps, please let me know if you need any additional information or assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"