人Heat Shock Protein 47 ELISA试剂盒(SERPINH1) (ab108861)

概述

  • 产品名称人Heat Shock Protein 47 ELISA试剂盒(SERPINH1)
    参阅全部 Hsp 47 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Milk, Serum, Plasma, Tissue, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    > 0.9 ng/ml
  • 范围
    1.563 ng/ml - 50 ng/ml
  • 复检

    96 %

  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human cell culture lysates, milk, serum, plasma and tissue extract.


    A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 疾病相关Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10) [MIM:613848]. A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae.
  • 序列相似性Belongs to the serpin family.
  • 细胞定位Endoplasmic reticulum lumen.
  • Information by UniProt
  • 别名
    • 47 kDa heat shock protein
    • Arsenic-transactivated protein 3
    • AsTP3
    • Cell proliferation-inducing gene 14 protein
    • Collagen-binding protein
    • Collagen-binding protein 1
    • Colligin
    • Rheumatoid arthritis-related antigen RA-A47
    • SERPH_HUMAN
    • Serpin H1
    • Serpin Peptidase Inhibitor
    • SERPINH1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) 图像

  • Representative Standard Curve using ab108861.

实验方案

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)参考文献

This product has been referenced in:
  • Fang JY  et al. Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics. Proteomics 16:2718-2731 (2016). Read more (PubMed: 27459910) »

See 1 Publication for this product

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