人Heat Shock蛋白47 ELISA试剂盒(SERPINH1) (ab108861)
Key features and details
- Sensitivity: 0.74 ng/ml
- Range: 1.563 ng/ml - 25 ng/ml
- Sample type: Cell Lysate, Cerebral Spinal Fluid, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Heat Shock蛋白47 ELISA试剂盒(SERPINH1)
参阅全部 Hsp47 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6.1% 批次间 样品 n Mean SD CV% Overall 10.9% -
样品类型
Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.74 ng/ml -
范围
1.563 ng/ml - 25 ng/ml -
回收率
98 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human plasma, milk, serum, cell lysate, cerebrospinal fluid and tissue samples.
A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.
Get results in 90 minutes with Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1 x 30ml 1X Standard Diluent 1 x 2ml 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 2 x 30ml 50X Biotinylated Human HSP47 Antibody 1 x 150µl 1 x 150µl Chromogen Substrate 1 x 7ml 1 x 7ml HSP47 Microplate (12 x 8 well strips) 1 unit 1 unit HSP47 Standard 1 vial 1 vial Sealing Tapes 3 units 3 units Stop Solution 1 x 11ml 1 x 11ml -
研究领域
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功能
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. -
疾病相关
Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. -
序列相似性
Belongs to the serpin family. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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别名
- 47 kDa heat shock protein
- 47 kDa heat shock protein precursor
- Arsenic transactivated protein 3
see all -
数据库链接
- Entrez Gene: 871 Human
- Omim: 600943 Human
- SwissProt: P50454 Human
- Unigene: 596449 Human
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108861 被引用在 2 文献中.
- Fang JY et al. Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics. Proteomics 16:2718-2731 (2016). PubMed: 27459910
- Chu H et al. Involvement of collagen-binding heat shock protein 47 in scleroderma-associated fibrosis. Protein Cell 6:589-598 (2015). PubMed: 26091621