人Heat Shock Protein 47 ELISA试剂盒(SERPINH1) (ab108861)

概述

  • 产品名称
    人Heat Shock Protein 47 ELISA试剂盒(SERPINH1)
    参阅全部 Hsp 47 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.6%
  • 样品类型
    Milk, Serum, Plasma, Tissue, Cell Lysate
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    > 0.9 ng/ml
  • 范围
    1.563 ng/ml - 50 ng/ml
  • 复检

    96 %

  • 检测时间
    5h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human cell culture lysates, milk, serum, plasma and tissue extract.


    A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.


    Get results in 90 minutes with Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108861.

实验方案

文献

This product has been referenced in:
  • Fang JY  et al. Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics. Proteomics 16:2718-2731 (2016). Read more (PubMed: 27459910) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your inquiry.

I heard back from our lab that for this kit, the antibodies were raised against recombinant proteins and the epitopes have not been mapped.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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