人Heat Shock Protein 47 ELISA试剂盒(SERPINH1) (ab108861)

概述

  • 产品名称人Heat Shock Protein 47 ELISA试剂盒(SERPINH1)
    参阅全部 Hsp 47 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.6%
  • 样品类型
    Milk, Serum, Plasma, Tissue, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    > 0.9 ng/ml
  • 范围
    1.563 ng/ml - 50 ng/ml
  • 复检

    96 %

  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Save time with a 90-minute ELISA protocol. Try Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA® range.


    Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human cell culture lysates, milk, serum, plasma and tissue extract.


    A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 疾病相关Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10) [MIM:613848]. A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae.
  • 序列相似性Belongs to the serpin family.
  • 细胞定位Endoplasmic reticulum lumen.
  • Information by UniProt
  • 别名
    • 47 kDa heat shock protein
    • Arsenic-transactivated protein 3
    • AsTP3
    • Cell proliferation-inducing gene 14 protein
    • Collagen-binding protein
    • Collagen-binding protein 1
    • Colligin
    • Rheumatoid arthritis-related antigen RA-A47
    • SERPH_HUMAN
    • Serpin H1
    • Serpin Peptidase Inhibitor
    • SERPINH1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) 图像

  • Representative Standard Curve using ab108861.

实验方案

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)参考文献

This product has been referenced in:
  • Fang JY  et al. Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics. Proteomics 16:2718-2731 (2016). Read more (PubMed: 27459910) »

See 1 Publication for this product

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