人Heat Shock Protein 27 ELISA试剂盒(HSP27) (ab113334)

概述

  • 产品名称人Heat Shock Protein 27 ELISA试剂盒(HSP27)
    参阅全部 Heat Shock Protein 27 (HSP27) 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.15 ng/ml
  • 范围
    0.205 ng/ml - 50 ng/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 133.1 123% - 151%
    Serum 126.9 117% - 134%
    Plasma 119.8 105% - 142%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Heat Shock Protein 27 (HSP27) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HSP27 in serum, plasma, cell culture supernatants.


    This assay employs an antibody specific for Human Heat Shock Protein 27 coated on a 96-well plate. Standards and samples are pipetted into the wells and Heat Shock Protein 27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Heat Shock Protein 27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Heat Shock Protein 27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    ave time with a 90-minute ELISA protocol. Try Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Involved in stress resistance and actin organization. Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm; cytoskeleton; spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • 别名
    • HSPB1
    • 28 kDa heat shock protein
    • CMT2F
    • estrogen regulated 24 kDa protein
    • Estrogen-regulated 24 kDa protein
    • Heat shock 27 kDa protein
    • heat shock 27kDa protein 1
    • heat shock protein beta 1
    • Heat shock protein beta-1
    • HMN2B
    • HS.76067
    • HSP 27
    • Hsp25
    • HSP27
    • HSP28
    • HspB1
    • SRP27
    • stress responsive protein 27
    • Stress-responsive protein 27
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Heat Shock Protein 27 ELISA Kit (HSP27) 图像

  • Representative Standard Curve using ab113334

实验方案

Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab113334)参考文献

ab113334 has not yet been referenced specifically in any publications.

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