人Heat Shock Protein 27 ELISA试剂盒(HSP27) (ab108862)

概述

  • 产品名称人Heat Shock Protein 27 ELISA试剂盒(HSP27)
    参阅全部 Heat Shock Protein 27 (HSP27) 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.6%
    批次间
    样品 n Mean SD CV%
    Overall 7.3%
  • 测试
    1 x 96 well plate
  • 样品类型
    Milk, Serum, Plasma, Cell culture extracts, Tissue
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    > 0.3 ng/ml
  • 范围
    0.313 ng/ml - 80 ng/ml
  • 复检

    97 %

  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.

    A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Involved in stress resistance and actin organization. Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm; cytoskeleton; spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • 别名
    • HSPB1
    • 28 kDa heat shock protein
    • CMT2F
    • estrogen regulated 24 kDa protein
    • Estrogen-regulated 24 kDa protein
    • Heat shock 27 kDa protein
    • heat shock 27kDa protein 1
    • heat shock protein beta 1
    • Heat shock protein beta-1
    • HMN2B
    • HS.76067
    • HSP 27
    • Hsp25
    • HSP27
    • HSP28
    • HspB1
    • SRP27
    • stress responsive protein 27
    • Stress-responsive protein 27
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 27 ELISA Kit (HSP27) 图像

  • Representative Standard Curve using ab108862.

实验方案

Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab108862)参考文献

This product has been referenced in:
  • White NM  et al. Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma. Oncotarget 5:506-18 (2014). Read more (PubMed: 24504108) »
  • Enomoto M  et al. Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators. Am J Physiol Lung Cell Mol Physiol 306:L207-15 (2014). WB . Read more (PubMed: 24242011) »

See all 3 Publications for this product

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