人Heat Shock Protein 27 ELISA试剂盒(HSP27) (ab108862)

概述

  • 产品名称
    人Heat Shock Protein 27 ELISA试剂盒(HSP27)
    参阅全部 Heat Shock Protein 27 (HSP27) 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.6%
    批次间
    样品 n Mean SD CV%
    Overall 7.3%
  • 样品类型
    Milk, Serum, Plasma, Cell culture extracts, Tissue
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    > 0.3 ng/ml
  • 范围
    0.313 ng/ml - 80 ng/ml
  • 复检

    97 %

  • 检测时间
    5h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.


    A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.


    Get results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108862.

实验方案

文献

This product has been referenced in:
  • White NM  et al. Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma. Oncotarget 5:506-18 (2014). Read more (PubMed: 24504108) »
  • Enomoto M  et al. Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators. Am J Physiol Lung Cell Mol Physiol 306:L207-15 (2014). WB . Read more (PubMed: 24242011) »

See all 3 Publications for this product

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