概述

  • 产品名称
    人HDL ELISA试剂盒
    参阅全部 HDL 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 样品类型
    Cell culture supernatant, Milk, Serum, Plasma
  • 检测类型
    Competitive
  • 灵敏度
    6 µg/ml
  • 范围
    6.25 µg/ml - 400 µg/ml
  • 复检

    96.5 %

  • 检测时间
    3h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s High-Density Lipoprotein (HDL) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human HDL in plasma, serum, milk, and cell culture supernatant.

    A HDL specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated HDL is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of HDL captured in plate.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

  • 相关性

    High-density lipoprotein (HDL) is one of the five major groups of lipoproteins, which, in order of sizes, largest to smallest, are chylomicrons, VLDL, IDL, LDL, and HDL, which enable lipids like cholesterol and triglycerides to be transported within the water-based bloodstream. In healthy individuals, about thirty percent of blood cholesterol is carried by HDL.

    Blood tests typically report HDL-C level, i.e. the amount of cholesterol contained in HDL particles. It is often contrasted with low-density or LDL cholesterol or LDL-C. HDL particles are able to remove cholesterol from within artery atheroma and transport it back to the liver for excretion or re-utilization, which is the main reason why the cholesterol carried within HDL particles (HDL-C) is sometimes called "good cholesterol" (despite the fact that it is exactly the same as the cholesterol in LDL particles).

  • 别名
    • High Density Lipoprotein

应用

Our Abpromise guarantee covers the use of ab125961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Human HDL ELISA Kit 图像

  • Representative Standard Curve using ab125961

实验方案

Human HDL ELISA Kit (ab125961)参考文献

This product has been referenced in:
  • Ito F  et al. The Application of a Modified d-ROMs Test for Measurement of Oxidative Stress and Oxidized High-Density Lipoprotein. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28230785) »

See 1 Publication for this product

Product Wall

The ab125961 Human HDL ELISA Kit antibodies are polyclonal antibody against total HDL and recognize lipoprotein in the HDL.  It has some cross reactivity with Lp(a).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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