概述

  • 产品名称
    人HDL ELISA试剂盒
    参阅全部 HDL 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 样品类型
    Cell culture supernatant, Milk, Serum, Plasma
  • 检测类型
    Competitive
  • 灵敏度
    6 µg/ml
  • 范围
    6.25 µg/ml - 400 µg/ml
  • 复检

    96.5 %

  • 检测时间
    3h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s High-Density Lipoprotein (HDL) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human HDL in plasma, serum, milk, and cell culture supernatant.

    A HDL specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated HDL is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of HDL captured in plate.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab125961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab125961

实验方案

文献

This product has been referenced in:
  • Ito F  et al. The Application of a Modified d-ROMs Test for Measurement of Oxidative Stress and Oxidized High-Density Lipoprotein. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28230785) »

See 1 Publication for this product

客户评价及客户问答

The ab125961 Human HDL ELISA Kit antibodies are polyclonal antibody against total HDL and recognize lipoprotein in the HDL.  It has some cross reactivity with Lp(a).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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