概述

  • 产品名称人hCG ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    54.87 pg/ml - 40000 pg/ml
  • 复检

    > 90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 130 120% - 135%
    Serum 113.7 103% - 124%
    Plasma 90.57 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s hCG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human hCG in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human hCG coated on a 96-well plate. Standards and samples are pipetted into the wells and hCG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human hCG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of hCG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
  • 别名
    • Anterior pituitary glycoprotein hormones common subunit alpha
    • CG ALPHA
    • CG beta
    • CG beta a
    • CGA
    • CGB
    • CGB1
    • CGB2
    • CGB3
    • CGB5
    • CGB7
    • CGB8
    • Choriogonadotropin alpha chain
    • Choriogonadotropin subunit beta
    • Chorionic gonadotrophin alpha subunit
    • Chorionic gonadotrophin chain beta
    • Chorionic gonadotropin alpha polypeptide
    • Chorionic gonadotropin beta 5 subunit
    • Chorionic gonadotropin beta 7 subunit
    • Chorionic gonadotropin beta 8 subunit
    • Chorionic gonadotropin beta chain
    • Chorionic gonadotropin beta polypeptide
    • Chorionic gonadotropin beta polypeptide 1
    • Chorionic gonadotropin beta polypeptide 2
    • Chorionic gonadotropin beta polypeptide 5
    • Chorionic gonadotropin beta polypeptide 7
    • Chorionic gonadotropin beta subunit
    • Follicle stimulating hormone alpha chain
    • Follitropin alpha chain
    • FSH alpha
    • FSHA
    • Glycoprotein hormones alpha chain precursor
    • Glycoprotein hormones alpha polypeptide
    • GPHa
    • GPHA 1
    • GPHA1
    • hCGB
    • Human Chorionic Gonadotropin
    • LHA
    • LSH alpha
    • Luteinizing hormone alpha chain
    • Lutropin alpha chain
    • Thyroid stimulating hormone alpha chain
    • Thyrotropin alpha chain
    • TSH alpha
    • TSHA
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human hCG ELISA Kit 图像

  • Representative standard curve using ab100533

  • Representative standard curve using ab100533

实验方案

Human hCG ELISA Kit (ab100533)参考文献

This product has been referenced in:
  • Sivasubramaniyam T  et al. Where polarity meets fusion: role of Par6 in trophoblast differentiation during placental development and preeclampsia. Endocrinology 154:1296-309 (2013). Read more (PubMed: 23341197) »
  • Nagirnaja L  et al. Structural and functional analysis of rare missense mutations in human chorionic gonadotrophin ß-subunit. Mol Hum Reprod 18:379-90 (2012). Read more (PubMed: 22554618) »

See all 2 Publications for this product

Product Wall

Vielen Dank für Ihre Anfrage.

Ich habe um mehr Informationen aus dem Labor gebeten und freue mich Ihnen folgendes bestätigen zu können:

1.) Dieses Kit ist getestet intaktes humanes hCG zu erkennen.

2.) Beide...

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Thank you for your enquiry.
I can confirm that both the capture and detection antibodies used in ab100533 hCG Human ELISA Kit are monoclonal.
I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

Unfortunately the components of this kit are considered proprioetary by the lab and as such I do not have the information which you are requesting. However we do offer hCG antibody pairs which are tested and guaranteed in sELISA. The best recommended...

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Thank you for contacting us.

The kit ab100533 is intenteded to detect hCG (intact). If you're looking just to detect hCG beta, you may be interested in ab108638.

http://www.abcam.com/chorionic-gonadotropin-beta-human-elisa-kit-ab10...

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1. You can purchase this kit directly from us, Abcam is based in Cambridge, England and this will ship directly from that office to you. 2. You do not have to use the same standard dilution scheme in the manual if you do not want.  However, the sen...

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Thank you for your enquiry. I can confirm that the hCG Human ELISA Kit detects the intact hCG molecule (heterodimer). The capture antibody is a mouse monoclonal and recognizes the alpha subunit (hCG-a). The detection antibody is also a mouse monocl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"