人HB EGF ELISA试剂盒(ab100531)

概述

  • 产品名称人HB EGF ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    16.38 pg/ml - 4000 pg/ml
  • 复检

    90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 81.22 73% - 90%
    Serum 99.18 89% - 108%
    Plasma 93.73 84% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in serum, plasma, and cell culture supernatants. (Human HB EGF concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human HB EGF ELISA Kit 图像

  • Representative standard curve using ab100531

  • Representative standard curve using ab100531

实验方案

Human HB EGF ELISA Kit (ab100531)参考文献

ab100531 has not yet been referenced specifically in any publications.

Product Wall



1. Increase amount of biotinylated ab (by 1.5 fold or so – too much may increase background)
2. Incubate biotinylated ab overnight at 4 degrees C
3. Increase amount of HRP-strep (by about 1.5 fold or so – to...

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Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacell...

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Thank you for contacting us.

I am very sorry to hear these kits failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to ...

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Thank you for the updates.

We rarely recommend running plasma or serum samples undiluted, as matrix effects are more likely to occur. For this kit (ab100531: HB EGF Human ELISA Kit), we recommend a 2-fold dilution of plasma or serum. This may...

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Muchas gracias por contactarnos. Le pido disculpas por la espera.

El kit al que se refiere es valido para detectar HB EGF humano en suero, plasma y sobrenadante de cultivo celular. En el protocolo adjunto en la página web se indican lo...

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Thank you for the enquiry regarding ab100531 and for sending the customer’s data/comments.

Please find enclosed the attached file for the recent analysis. As you can see, the standard curves obtained have good linearity and very low CV%...

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Thank you for your enquiry regarding ab100531. I am very sorry to hear that your customer having problems with this kit.

The batch number you have provided (81802) does not match to our record. Could you please check it again and confirm the ...

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Thank you for your call yesterday and for your questions. I have been in touch with my colleague in the lab, and have received more information about ab100531.

The standard provided in ab100531 is expressed in baculovirus, and it corresonds t...

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Thank you for contacting Abcam.

Youshould use Assay Diluent C if diluting conditioned media samples.

Please letme knowif you haveany questions.

Thank you for contacting Abcam.

Please find the answers to your questions below:

1. are the coating antibody and the detection antibody a polyclonal or
monoclonal antibodies?Capture antibody = monoclonal
Detection antibody...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"