人Haptoglobin ELISA试剂盒(ab108856)
Key features and details
- Sensitivity: 57 ng/ml
- Range: 0.078 µg/ml - 20 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
-
产品名称
人Haptoglobin ELISA试剂盒
参阅全部 Haptoglobin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6.1% 批次间 样品 n Mean SD CV% Overall 10.54% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
= 57 ng/ml -
范围
0.078 µg/ml - 20 µg/ml -
回收率
= 90 %
-
检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human Haptoglobin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.
A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Haptoglobin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Human Haptoglobin (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 7ml Haptoglobin Microplate (12 x 8 well strips) 1 unit Haptoglobin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
-
功能
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
组织特异性
Expressed by the liver and secreted in plasma. -
疾病相关
Anhaptoglobinemia -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Secreted. - Information by UniProt
-
别名
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
数据库链接
- Entrez Gene: 3240 Human
- Omim: 140100 Human
- SwissProt: P00738 Human
- Unigene: 513711 Human
- Unigene: 702099 Human
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab108856 被引用在 11 文献中.
- Muñoz-San Martín C et al. First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin. Anal Bioanal Chem 415:2045-2057 (2023). PubMed: 36344668
- Macarlupú JL et al. Sub-maximal aerobic exercise training reduces haematocrit and ameliorates symptoms in Andean highlanders with chronic mountain sickness. Exp Physiol 106:2198-2209 (2021). PubMed: 34555237
- Paul S et al. Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans. Funct Integr Genomics 20:191-200 (2020). PubMed: 31444657
- Tran TTT et al. APOE genotype influences the gut microbiome structure and function in humans and mice: relevance for Alzheimer's disease pathophysiology. FASEB J N/A:fj201900071R (2019). PubMed: 30958695
- Lv LL et al. Effect of haptoglobin on the treatment of chronic hepatitis B with interferon. Exp Ther Med 18:1417-1425 (2019). PubMed: 31316628