人GM-CSF Receptor alpha ELISA试剂盒(CD116) (ab46086)

概述

  • 产品名称人GM-CSF Receptor alpha ELISA试剂盒(CD116)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 31 700.2pg/ml 20.4 2.9%
    B 32 378pg/ml 12.8 3.4%
    批次间
    样品 n Mean SD CV%
    A 6 555pg/ml 22.2 4%
    B 6 120.7pg/ml 2.2 1.8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 16 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s GM-CSF Receptor alpha (CD116) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF R alpha in serum, plasma, and cell culture supernatants.

    A monoclonal antibody specific for GM-CSF R alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF R alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF R alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF R alpha present in the samples.

    This kit will recognize both endogenous and recombinant Human GM-CSF R alpha.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-GM-CSF R alpha Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    GM-CSF Receptor alpha Microplate (12 x 8 well strips) 1 unit 2 units
    GM-CSF Receptor alpha Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性GM-CSF Receptor alpha is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.
  • 细胞定位Cell membrane; single-pass type I membrane protein. Isoform 3, isoform 4, isoform 6: Secreted protein
  • 别名
    • CD116
    • CD116 antigen
    • CDw116
    • Colony stimulating factor 2 receptor alpha low affinity (granulocyte macrophage)
    • CSF2R
    • CSF2RAX
    • CSF2RAY
    • CSF2RX
    • CSF2RY
    • GM CSF R alpha
    • GMCSFR
    • GMR
    • Granulocyte macrophage colony stimulating factor receptor alpha chain
    • MGC3848
    • MGC4838
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human GM-CSF Receptor alpha ELISA Kit (CD116) 图像

  • Representative Standard Curve using ab46086

实验方案

Human GM-CSF Receptor alpha ELISA Kit (CD116) (ab46086)参考文献

ab46086 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The information available concerning this kit suggests that it will still recognize the GM-CSF Receptor when bound to the related cytokine (GM-CSF). This has not been verified by our lab. I hope this information is...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"