人GITR Ligand ELISA试剂盒(TNFSF18) (ab113323)

概述

  • 产品名称
    人GITR Ligand ELISA试剂盒(TNFSF18)
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    41 pg/ml - 10000 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 87.83 67% - 100%
    Serum 116.9 108% - 123%
    Plasma 87.69 67% - 100%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s GITR Ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GITR Ligand in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human GITR Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and GITR Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GITR Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GITR Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.
  • 组织特异性
    Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • Activation inducible TNF related ligand
    • Activation-inducible TNF-related ligand
    • AITR ligand
    • AITRL
    • GITR ligand
    • GITRL
    • Glucocorticoid induced TNF related ligand
    • Glucocorticoid induced TNFR related protein ligand
    • Glucocorticoid-induced TNF-related ligand
    • hGITRL
    • MGC138237
    • TL6
    • TNF18_HUMAN
    • TNFSF18
    • TNLG2A
    • Tumor necrosis factor (ligand) superfamily member 18
    • Tumor necrosis factor ligand 2A
    • Tumor necrosis factor ligand superfamily member 18
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human GITR Ligand ELISA Kit (TNFSF18) 图像

  • Representative standard curve using ab113323

实验方案

Human GITR Ligand ELISA Kit (TNFSF18) (ab113323)参考文献

ab113323 has not yet been referenced specifically in any publications.

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