人GC Globulin ELISA试剂盒(Vitamin D Binding Protein) (ab108853)

概述

  • 产品名称人GC Globulin ELISA试剂盒(Vitamin D Binding Protein)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型Competitive
  • 灵敏度
    > 0.09 µg/ml
  • 范围
    0.098 µg/ml - 100 µg/ml
  • 复检

    98.5 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma, serum, saliva, milk and cell-culture supernatants.

    A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • DBP
    • GC
    • Gc-globulin
    • Group-specific component
    • VDB
    • Vitamin D-binding protein
    • VTDB_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

Human GC Globulin ELISA Kit (Vitamin D Binding Protein) 图像

  • Representative Standard Curve using ab108853.

实验方案

Human GC Globulin ELISA Kit (Vitamin D Binding Protein) (ab108853)参考文献

ab108853 has not yet been referenced specifically in any publications.

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