人GAPDH exon 1 ChIP引物and Probe Set (ab85378)

概述

  • 产品名称
    人GAPDH exon 1 ChIP引物and Probe Set
    参阅全部 GAPDH 试剂盒
  • 产品概述

    Accession number: NM_002046.
    The HsGAPDH primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells.

    GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) is a highly expressed housekeeping gene. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most cell types, this locus is highly enriched with histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441). Whereas it shows low levels of histone modifications associated with silencing such as Histone H3 K9 tri methylation (see image with ab71604).

性能

文献

ab85378 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry.

I can confirm thatany of the loci we use for positive and negative controlsin the ChIP image on the datasheet would be good to use.In this case for example, we expect a strong signal in the active loci (GAPDH, RPL...

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Thank you for your enquiry and your response.

This is recommended and normally it is in their standard Taqman protocol as the first step. This step is to get all reagents at the same temperature.

I hope this will be useful for you.

Thank you for your interest.

Further to my previous e-mail, please findenclosed some more information about the primers which are in the Abcam catalogue and can be considered using. I would advise you to take a look at this site: http://www.a...

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Thank you for your reply. I have confirmed with our laboratory that the primer pairs were specifically generated for the human sequences.  We have not tested them with mouse and therefore cannot confirm that they give a single, specific PCR product...

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