SimpleStep

人gamma-synuclein ELISA试剂盒(ab224884)

概述

  • 产品名称
    人gamma-synuclein ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Serum 8 6.1%
    批次间
    样品 n Mean SD CV%
    Serum 3 14%
  • 样品类型
    Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • 检测类型
    Semi-quantitative
  • 灵敏度
    5.15 pg/ml
  • 范围
    7.81 pg/ml - 500 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Urine 107 100% - 114%
    Serum 100 90% - 105%
    Heparin Plasma 89 83% - 92%
    EDTA Plasma 73 66% - 77%
    Citrate Plasma 79 75% - 84%

  • 检测时间
    1h 30m
  • 实验步骤
    One step assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Gamma-synuclein in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Gamma-synuclein protein in Human  Serum, Plasmas, Urine and Cell Extracts


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Human gamma-synuclein plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.


    Sensitivity


    Samples diluted in Sample Diluent NS - 5.23 ng/mL


    Samples diluted in Sample Diluent PTR - 5.15 ng/mL

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Pre-coated microplate (12 x 8 well strips)

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    Human gamma-synuclein Capture Antibody 10X 1 x 600µl
    Human gamma-synuclein Detector Antibody 10X 1 x 600µl
    Human gamma-synuclein Lyophilized Recombinant 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • 研究领域
  • 功能
    Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
  • 组织特异性
    Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
  • 序列相似性
    Belongs to the synuclein family.
  • 翻译后修饰
    Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
  • 细胞定位
    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
  • Information by UniProt
  • 别名
    • BCSG1
    • Breast cancer specific gene 1 protein
    • Breast cancer specific protein 1
    • Breast cancer-specific gene 1 protein
    • Gamma synuclein
    • Gamma-synuclein
    • Persyn
    • PRSN
    • SNCG
    • SR
    • Synoretin
    • Synuclein gamma
    • synuclein, gamma (breast cancer-specific protein 1)
    • SYUG_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab224884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • The gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of gamma-synuclein were measured in duplicate, interpolated from the gamma-synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 126.89 pg/mL in serum, 114.40 pg/mL in plasma (citrate), 78.63 pg/mL in plasma (EDTA) and 173.43 pg/ml in plasma (heparin).

  • The concentrations of gamma-synuclein were measured in duplicate and interpolated from the gamma-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 2193.46 pg/mL in bladder cancer urine, 316.85 pg/ml in HT-29 cell extract.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 180.4 pg/mL with a range of 32.92 - 451.67 pg/mL.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 569.88 pg/mL with a range of 40.46 – 2021.56 pg/ml. The native concentration of gamma-synuclein was below the level of detection in urine from healthy human donors.

实验方案

文献

ab224884 has not yet been referenced specifically in any publications.

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