人Galectin 7 ELISA试剂盒(GAL7) (ab100523)

概述

  • 产品名称人Galectin 7 ELISA试剂盒(GAL7)
    参阅全部 Galectin 7 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    13.72 pg/ml - 10000 pg/ml
  • 复检

    > 90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.55 87% - 105%
    Serum 107.1 95% - 115%
    Plasma 91.82 70% - 125%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Galectin 7 (GAL7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Galectin 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Galectin 7 is thought to be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Galectin 7 has been implicated in apoptosis and is believed to upregulate MMP9 and as such be involved in the progression of tumorigenesis.
  • 别名
    • GAL7
    • HKL14
    • Human keratinocyte lectin 14
    • Lectin galactoside binding soluble 7
    • LGALS7
    • LGALS7B
    • MGC151215
    • MGC151217
    • OTTMUSP00000025970
    • OTTMUSP00000038956
    • p53 induced gene 1 protein
    • PI7
    • PIG1
    • TP53I1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Galectin 7 ELISA Kit (GAL7) 图像

  • Representative Standard Curve using ab100523.

  • Representative Standard Curve using ab100523.

实验方案

Human Galectin 7 ELISA Kit (GAL7) (ab100523)参考文献

ab100523 has not yet been referenced specifically in any publications.

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I have had the opportunity to speak with the lab, unfortunately, the reagents used in the diluents and buffers are proprietary and therefore unavailable. They recommend you use the wash buffer provided (Item ...

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Thank you for contacting us. Although the immunogen sequence for this antibody is commercially available, I performed a BLAST between the whole human protein sequence and the whole mouse protein sequence. The percentage of homology obtained is ...

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