概述
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产品名称人G-CSF ELISA试剂盒
参阅全部 G-CSF 试剂盒 -
检测方法Colorimetric
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样品类型Cell culture supernatant, Serum, Plasma
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检测类型Sandwich (quantitative)
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灵敏度< 1 pg/ml
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范围0.69 pg/ml - 500 pg/ml
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回收率
> 75 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 76.56 68% - 90% Serum 83.45 72% - 94% Plasma 74.76 67% - 87% -
实验步骤Multiple steps standard assay
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种属反应性与反应: Human
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产品概述
Abcam’s G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human G-CSF ELISA Kit (CSF3) (ab188390) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples
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经测试应用适用于: Sandwich ELISAmore details
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平台Microplate
性能
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存放说明Store at -20°C. Please refer to protocols.
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组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 260X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human G-CSF (lyophilized) 2 vials G-CSF Microplate (12 x 8 wells) 1 unit Recombinant Human G-CSF Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions.
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别名
- C17orf33
- Colony stimulating factor 3 (granulocyte)
- CSF 3
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数据库链接
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- Unigene: 2233 Human
相关产品
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SimpleStep ELISA kits
应用
Our Abpromise guarantee covers the use of ab100524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
图片
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ELISA:G-CSF Human ELISA Kit (ab100524)Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).
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ELISA:G-CSF Human ELISA Kit (ab100524)Human G-CSF measured in undiluted culture supernatants (duplicates +/- SD).
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Typical standard curveRepresentative standard curve using ab100524
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ELISA:G-CSF Human ELISA Kit (ab100524)G-CSF measured in undiluted human plasma, signal in serum was below level of detection (duplicates +/- SD).
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Typical standard curveRepresentative standard curve using ab100524
实验方案
文献
ab100524 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"