> 75
%
样品类型 | 平均% | 范围 |
---|---|---|
Cell culture supernatant | 76.56 | 68% - 90% |
Serum | 83.45 | 72% - 94% |
Plasma | 74.76 | 67% - 87% |
Abcam’s G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human G-CSF ELISA Kit (CSF3) (ab188390) from our SimpleStep ELISA® range.
Optimization may be required with urine samples
组件 | 1 x 96 tests |
---|---|
20X Wash Buffer Concentrate | 1 x 25ml |
260X HRP-Streptavidin Concentrate | 1 x 200µl |
5X Assay Diluent B | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-Human G-CSF (lyophilized) | 2 vials |
G-CSF Microplate (12 x 8 wells) | 1 unit |
Recombinant Human G-CSF Standard | 2 vials |
Stop Solution | 1 x 8ml |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).
Human G-CSF measured in undiluted culture supernatants (duplicates +/- SD).
Representative standard curve using ab100524
G-CSF measured in undiluted human plasma, signal in serum was below level of detection (duplicates +/- SD).
Representative standard curve using ab100524
ab100524 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"