人Furin ELISA试剂盒(ab113322)

概述

  • 产品名称人Furin ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    13.72 pg/ml - 10000 pg/ml
  • 复检

    84 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 89.57 80% - 98%
    Plasma 77.74 69% - 85%
    Cell culture media 84.69 75% - 93%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Furin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Furin in serum, plasma (collect plasma using heparin and EDTA as an anticoagulant. Citrate is not recommended) and cell culture supernatants.

    This assay employs an antibody specific for Human Furin coated on a 96-well plate. Standards and samples are pipetted into the wells and Furin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Furin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Furin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Furin ELISA Kit 图像

  • Representative standard curve using ab113322

  • Representative standard curve using ab113322

实验方案

Human Furin ELISA Kit (ab113322)参考文献

ab113322 has not yet been referenced specifically in any publications.

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This kit has not been designed nor validated with tissue lysate samples. However it is very accommodating to many different samples types, so lysates will most likely work. If you decide to test this kit with tiss...

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