人Free Estriol ELISA试剂盒(ab108671)

概述

  • 产品名称人Free Estriol ELISA试剂盒
    参阅全部 Estriol 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.05 ng/ml
  • 范围
    0.1 ng/ml - 12 ng/ml
  • 复检

    101.6 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Free Estriol competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Free Estriol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Estriol IgG. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 别名
    • Oestriol

应用

Our Abpromise guarantee covers the use of ab108671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

Human Free Estriol ELISA Kit 图像

  • Representative Standard Curve using ab108671.

实验方案

Human Free Estriol ELISA Kit (ab108671)参考文献

ab108671 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We have a number of antibodies directed against estriol, ab64546, ab20278 and ab44962 are sheep, mouse and rat polyclonals respectively. These have all been tested with radioimmunoassay. Unfortunately we do not have...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"