人Fractalkine ELISA试剂盒(CX3CL1) (ab100522)
Key features and details
- Sensitivity: 300 pg/ml
- Range: 0.25 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Fractalkine ELISA试剂盒(CX3CL1)
参阅全部 CX3CL1 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 300 pg/ml -
范围
0.25 ng/ml - 60 ng/ml -
回收率
93 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94.47 83% - 102% Plasma 93.54 83% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CX3CL1 (Fraktaline) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CX3CL1 in plasma and cell culture supernatants. (Human CX3CL1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for human CX3CL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Fractalkine in normal serum with ab100522, only in serum spiked with Human Fractalkine.
Get higher sensitivity in only 90 minutes with Human Fractalkine ELISA Kit (CX3Cl1) (ab192145) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 320X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CX3CL1 2 vials CX3CL1 Microplate (12 x 8 well strips) 1 unit Recombinant Human CX3CL1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion. -
别名
- A 152E5.2
- AB030188
- ABCD 3
see all -
数据库链接
- Entrez Gene: 6376 Human
- Omim: 601880 Human
- SwissProt: P78423 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100522 被引用在 2 文献中.
- Guo C et al. CX3CL1 Derived from Bone Marrow Mesenchymal Stem Cells Inhibits Aβ1-42-Induced SH-SY5Y Cell Pathological Damage through TXNIP/NLRP3 Signaling Pathway. Comput Math Methods Med 2022:1949344 (2022). PubMed: 36118839
- Wojdasiewicz P et al. Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis. J Immunol Res 2020:2932696 (2020). PubMed: 32884948