人Flt3 Ligand ELISA试剂盒(ab100521)

概述

  • 产品名称人Flt3 Ligand ELISA试剂盒
    参阅全部 Flt3 ligand 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    4.1 pg/ml - 400 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 97.62 85% - 108%
    Serum 95.48 84% - 107%
    Plasma 94.39 83% - 106%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Flt3 Ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Flt3 Ligand in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Flt3 Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Flt3 Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Flt3 ligand is a ligand for the FLT3 tyrosine kinase receptor and belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3 ligand have been identified. The predominant form is the transmembrane form, which is biologically active on the cell surface. When proteolytically cleaved the transmembrane isoform generates a soluble form, which is also biologically active. Flt3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3 ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation.
  • 别名
    • Flt 3L
    • Flt3 L
    • FLT3 LG
    • Flt3L
    • FLT3LG
    • Fms related tyrosine kinase 3 ligand
    • SL cytokine
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Flt3 Ligand ELISA Kit 图像

  • Representative Standard Curve using ab100521.

  • Representative Standard Curve using ab100521.

实验方案

Human Flt3 Ligand ELISA Kit (ab100521)参考文献

ab100521 has not yet been referenced specifically in any publications.

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Thank you very much for sending the details. These were very helpful.

As we discussed on phone please try the experiment again by keeping all reagents at room temperature.

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As discussed I will be followingthis case further. Could you please provide the following details;

- Types of sample used. If supernatant, what type of cells were incubated
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