概述

  • 产品名称人FLRG ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 250 pg/ml
  • 范围
    204.8 pg/ml - 50000 pg/ml
  • 复检

    89 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.6 67% - 130%
    Serum 95.75 84% - 103%
    Plasma 77.86 67% - 99%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s FLRG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human FLRG coated on a 96-well plate. Standards and samples are pipetted into the wells and FLRG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FLRG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FLRG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human FLRG ELISA Kit 图像

  • Representative standard curve using ab113318

实验方案

Human FLRG ELISA Kit (ab113318)参考文献

ab113318 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"