概述
- 产品名称人Fibrinogen ELISA试剂盒
参阅全部 Fibrinogen 试剂盒 - 检测方法Colorimetric
- 精确度
批次内 样品 n Mean SD CV% Overall 4.9% 批次间 样品 n Mean SD CV% Overall 7.5% - 样品类型Plasma
- 检测类型Competitive
- 灵敏度= 0.16 µg/ml
- 范围0.165 µg/ml - 40 µg/ml
- 复检
98.5 %
- 检测时间3h 00m
- 实验步骤Multiple steps standard assay
- 种属反应性与反应: Human
- 产品概述
Abcam’s Fibrinogen Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen levels in plasma.
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.
Get better reproducibility in only 90 minutes with Human Fibrinogen ELISA Kit (ab208036) from our SimpleStep ELISA® range.
- 经测试应用适用于: Competitive ELISAmore details
- 平台Microplate
性能
- 存放说明Store at -20°C. Please refer to protocols.
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组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Human Fibrinogen (Lyophilized) 1 vial Chromogen Substrate 1 x 8ml Fibrinogen Microplate (12 x 8 well strips) 1 unit Fibrinogen Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml - 研究领域
- 功能Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
- 组织特异性Plasma.
- 疾病相关Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. - 序列相似性Contains 1 fibrinogen C-terminal domain.
- 结构域A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
- 翻译后修饰The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. - 细胞定位Secreted.
- Information by UniProt
-
别名
- FGA
- Fib2
- FIBA_HUMAN
see all -
数据库链接
- Entrez Gene: 2244 Human
- Entrez Gene: 2266 Human
- Entrez Gene: 2243 Human
- Omim: 134820 Human
- Omim: 134850 Human
- Omim: 134830 Human
- SwissProt: P02671 Human
- SwissProt: P02675 Human
see all
相关产品
- SimpleStep ELISA kits
应用
Our Abpromise guarantee covers the use of ab108842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Human Fibrinogen ELISA Kit 图像
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Sandwich ELISA - ab108842 Fibrinogen Human ELISA KitFibrinogen measured in various samples showing quantity (micrograms) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab108842.
实验方案
Human Fibrinogen ELISA Kit (ab108842)参考文献
ab108842 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"