概述

  • 产品名称人Fibrinogen ELISA试剂盒
    参阅全部 Fibrinogen 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4%
    批次间
    样品 n Mean SD CV%
    Overall 8.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 1 ng/ml
  • 范围
    1.25 ng/ml - 80 ng/ml
  • 复检

    96 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Fibrinogen Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of fibrinogen concentrations in cell culture media, saliva, milk, and urine samples.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.


    For higher reproducibility in only 90 minutes, we recommend our SimpleStep ELISA® range made with recombinant monoclonal antibodies. Try Human Fibrinogen ELISA Kit (ab208036).

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • 组织特异性Plasma.
  • 疾病相关Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 序列相似性Contains 1 fibrinogen C-terminal domain.
  • 结构域A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • 翻译后修饰The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • FGA
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Fibrinogen ELISA Kit 图像

  • Representative Standard Curve using ab108841.

实验方案

Human Fibrinogen ELISA Kit (ab108841)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Cross reactive with Rhesus EDTA plasma samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
An experience laboratory technician completed this Fibrinogen ELISA from Abcam, catalog number AB108841, lot GR245646-10. The goal of the experiment was to determine the cross-reactivity of this kit for EDTA-prepared plasma samples and serum samples for M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius.

Overall, the kit, like other Abcam ELISA kits, was easy to follow and straight-forward. The laboratory technician had no problem understanding any parts of the protocol.

Our test was performed in 2 basic steps. The first was to find the appropriate testable dilution in plasma and serum, and the second was to test the linearity and spike recovery.

To this end our first experiment tested 8 dilutions of both serum and plasma ranging from 1:10 to 1:640 (8 1:2 serial dilutions). In these dilution ranges, the serum was well within the OD range, however no linearity was observed in any part of the dilution scheme. The EDTA plasma was beyond the range of detection (under-diluted).

Our second experiment focused on the EDTA prepared plasma. We diluted n=2 rhesus plasma samples 1:10,000, 1:30,000, 1:90,000, and 1:270,000 and tested in duplicate (as were the standards). The rest of the assay was performed to protocol’s specifications. For both animals tested, 1:90,000 and 1:270,000 gave similar results, suggesting this is the linear range of the assay.

Absorbance measurements of the diluted samples were comparable between duplicates in both tests (pipetting error appeared to be at a minimum). We also performed a spike recovery in both pure diluent and diluted plasma. ~100% of spiked human Fibrinogen was detected in the 90,000 and 270,000 diluted samples.

Overall, we recommend this kit for detection of M. mulatta fibrinogen in EDTA-prepared plasma.
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提交于 Jun 07 2016

Plasma treated with EDTA anticoagulant can be used with all three of these ELISA kits. However, if choosing between ab108842 and ab108841, the competitive ELISA ab108842 is preferable to ab108841 for this sample type. The SimpleStep ELISA ab171578 is p...

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For this lot, there are 400 ng of standard per vial. So, reconstitute in 5 mL of the diluent for a 80 ng/mL standard solution.

Thank you for you for your enquiry and your interest in our products.

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